Anti-Homeobox protein NANOG NANOG Antibody

About this product

Boster Bio Anti-Homeobox protein NANOG NANOG Antibody (Catalog # A00153) . Tested in ELISA, WB, IHC-P, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00153

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

Synonyms

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog

Gene Name

NANOG

UniProt

Q9H9S0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG. The immunogen is located within amino acids 130 - 180 of NANOG.

Clonality

Polyclonal

Tissue Specificity

Expressed in testicular carcinoma and derived germ cell tumors (at protein level) . Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

Applications

ELISA, WB, IHC-P, IF, ICC

Field of Research

Homeostasis, Stem Cells

Purification

NANOG Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768) . Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Storage Conditions

NANOG antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

34620 MW

Gene Name Synonym

Nanog homeobox

Subcellular Location

Nucleus

Prediction Reactivity

Bovine, Chicken

Protein Name

Nanog homeobox

Isotype

IgG

Contents

NANOG Antibody is supplied in PBS containing 0.02% sodium azide.