Anti-Interferon regulatory factor 3 IRF3 Antibody

About this product

Boster Bio Anti-Interferon regulatory factor 3 IRF3 Antibody (Catalog # A00165) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A00165

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interferons (IFN) s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by IκB kinase-ε and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.

Synonyms

Interferon regulatory factor 3, IRF-3

Gene Name

IRF3

UniProt

Q14653

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

At least three isoforms of IRF3 are known to exist.

Immunogen

IRF3 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human IRF3. The immunogen is located within amino acids 330 - 380 of IRF3.

Clonality

Polyclonal

Tissue Specificity

Expressed constitutively in a variety of tissues.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Chemokines, Cytokines, Homeostasis, Inflammasomes

Purification

IRF3 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Key transcriptional regulator of type I interferon (IFN) -dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Storage Conditions

IRF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

47219 MW

Gene Name Synonym

Interferon regulatory factor 3

Subcellular Location

Cytoplasm

Prediction Reactivity

Bovine, Pig

Protein Name

Interferon regulatory factor 3

Isotype

IgG

Contents

IRF3 Antibody is supplied in PBS containing 0.02% sodium azide.