Anti-Dnmt1 Antibody

About this product

Boster Bio Anti-Dnmt1 Antibody catalog # A00172. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.

Specifications

Catalog number

A00172

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

DNA (cytosine-5) -methyltransferase 1; Dnmt1;2.1.1.37; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT; DNMT1; AIM, CXXC9, DNMT

Gene Name

DNMT1

UniProt

P26358

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant fusion protein of human DNMT1 (NP_001370.1) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.

Applications

WB, ICC, IF, IP

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306) . Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306) . Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306) . Promotes tumor growth (PubMed:24623306) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

183165 MW

Gene Name Synonym

DNA (cytosine-5) -methyltransferase 1

Subcellular Location

Nucleus

Protein Name

DNA (cytosine-5) -methyltransferase 1

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2