- Catalog number
- A00191
- Size / count
- 0.1 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- TET2, a member of the ten-eleven-translocation (TET) family of genes, is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. It is a candidate tumor suppressor gene reported to be mutated in approximately 14% of patients with JAK2V617F-positive myeloproliferative neoplasms, and can be mutated in other hematopoietic disorders such as myelodysplastic syndromes, acute myeloid leukemia, and chronic myelomonocytic leukemia. Analysis of the TET2 and JAK2 mutations in these neoplasms suggests that mutations in TET2 do not represent a predisposition for acquiring mutations in JAK2.
- Synonyms
- MDS, KIAA1546, Nbla00191, Methylcytosine dioxygenase TET2
- Gene Name
- TET2
- UniProt
- Q6N021
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- At least two isoforms of TET2 are known to exist; this antibody will detect both isoforms.
- Immunogen
- TET2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TET2. The immunogen is located within amino acids 20 - 70 of TET2.
- Clonality
- Polyclonal
- Tissue Specificity
- Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes.
- Applications
- ELISA, WB, IHC-P, IF
- Field of Research
- Cancer, Cell Cycle
- Purification
- TET2 Antibody is affinity chromatography purified via peptide column.
- Concentration
- 1 mg/mL
- Form
- Liquid
- Function
- Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5- hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5- formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC) . Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.
- Storage Conditions
- TET2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Observed Molecular Weight
- 223811 MW
- Gene Name Synonym
- Tet methylcytosine dioxygenase 2
- Prediction Reactivity
- Mouse
- Protein Name
- Tet methylcytosine dioxygenase 2
- Isotype
- IgG
- Contents
- TET2 Antibody is supplied in PBS containing 0.02% sodium azide.
