Anti-PINK1 Antibody

About this product

Boster Bio Anti-PINK1 Antibody (Catalog # A00201) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00201

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The PTEN-induced putative kinase 1 (PINK1) is a serine/threonine protein kinase that localizes to mitochondria and is thought to protect cells from stress-induced mitochondrial dysfunction (reviewed in 1) . PINK1 recruits the E3 ubiquitin ligase Parkin to mitochondria to initiate mitophagy, an autophagic process that clears damaged mitochondria within a cell (2) . PINK1 is cleaved by the mitochondrial protease PARL (3) . Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease (4) .

Synonyms

BRPK, PARK6BRPK

Gene Name

PINK1

UniProt

Q9BXM7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

PINK1 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will only detect the longer isoform. PINK1 antibody will detect the cleaved and uncleaved form of PINK1.

Immunogen

PINK1 antibody was raised against a 16 amino acid peptide near the amino terminus of human PINK1. The immunogen is located within amino acids 120 - 170 of PINK1.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Autophagy, Neuroscience

Purification

PINK1 antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN (PubMed:14607334, PubMed:15087508, PubMed:19229105, PubMed:19966284, PubMed:20404107, PubMed:20798600, PubMed:23620051, PubMed:23754282, PubMed:23933751, PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:24896179, PubMed:25527291) . Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2 (PubMed:23620051) . Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291) . Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (By similarity) .

Storage Conditions

PINK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Observed Molecular Weight

62769 MW

Gene Name Synonym

PTEN induced putative kinase 1

Subcellular Location

Mitochondrion outer membrane

Protein Name

PTEN induced putative kinase 1

Isotype

IgG

Contents

PINK1 antibody is supplied in PBS containing 0.02% sodium azide.