Anti-CD86 Antibody

About this product

Anti-CD86 Antibody

Specifications

Catalog number

A00220

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD86, also known as B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD80, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) . Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1) . Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2) .

Synonyms

CD86 molecule, B70, B7-2, B7.2, LAB72, CD28LG2

Gene Name

CD86

UniProt

P42082

Host

Rabbit

Reactivity

Human

Cross Reactivity

At least five isoforms of CD86 are known to exist; this antibody will detect all five isoforms.

Immunogen

CD86 antibody was raised against a peptide corresponding to 17 amino acids near the center of human CD86. The immunogen is located within amino acids 160 - 210 of CD86.

Clonality

Polyclonal

Tissue Specificity

Expressed on activated B-cells.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Cell Cycle, Cancer, Immunology

Purification

CD86 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation.

Storage Conditions

CD86 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

34666 MW

Gene Name Synonym

CD86 molecule

Subcellular Location

Cell membrane ; Single-pass type I membrane protein .

Prediction Reactivity

Rabbit, Mouse

Protein Name

CD86 molecule

Isotype

IgG

Contents

CD86 Antibody is supplied in PBS containing 0.02% sodium azide.