Anti-Tuberin TSC2 Antibody

About this product

Boster Bio Anti-Tuberin TSC2 Antibody (Catalog # A00229) . Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A00229

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. At least three isoforms of TSC2 exist.

Synonyms

LAM, TSC4, Tuberin, Tuberous sclerosis 2 protein

Gene Name

TSC2

UniProt

P49815

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

TSC2 (NT) was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TSC2. The immunogen is located within the first 50 amino acids of TSC2.

Clonality

Polyclonal

Tissue Specificity

Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.

Applications

ELISA, WB, ICC, IF

Field of Research

Signal Transduction

Purification

TSC2 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed:12271141, PubMed:28215400) . Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1 (PubMed:15340059) . May also play a role in microtubule-mediated protein transport (By similarity) . Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity) .

Storage Conditions

TSC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

200608 MW

Gene Name Synonym

Tuberous sclerosis 2

Subcellular Location

Cytoplasm. Membrane; Peripheral membrane protein. At steady state found in association with membranes.

Prediction Reactivity

Rat

Protein Name

Tuberous sclerosis 2

Isotype

IgG

Contents

TSC2 Antibody is supplied in PBS containing 0.02% sodium azide.