Anti-Vimentin Antibody

About this product

Boster Bio Anti-Vimentin Antibody (Catalog # A00235-1) . Tested in WB, IF, Flow Cytometry, IHC-P-Leica application (s) . This antibody reacts with Human, Mouse.

Specifications

Catalog number

A00235-1-400ul

Size / count

400 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL) -derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Synonyms

Vimentin, VIM

Gene Name

VIM

UniProt

P08670

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This Vimentin antibody is generated from rabbits immunized with human Vimentin recombinant protein.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Applications

WB, IF, Flow Cytometry, IHC-P-Leica

Field of Research

Cancer, Developmental Biology, Neuroscience, Signal Transduction, Viral process, Host-Virus Interaction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

53652 Da

Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152}

Isotype

Rabbit IgG

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.