Anti-ARID1A Antibody

About this product

Boster Bio Anti-ARID1A Antibody (Catalog # A00247) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00247

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1) . It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2) .

Synonyms

AT rich interactive domain 1A (SWI-like), RP1-50O24.1, B120, BAF250, BAF250a, BM029, C1orf4, ELD, hELD, MRD14, OSA1, hOSA1, P270, SMARCF1

Gene Name

ARID1A

UniProt

O14497

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.

Immunogen

ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A. The immunogen is located within amino acids 580 - 630 of ARID1A.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Homeostasis, Signal Transduction

Purification

ARID1A antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology) . Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex) . During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF) . The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity) .

Storage Conditions

ARID1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Observed Molecular Weight

242045 MW

Gene Name Synonym

AT rich interactive domain 1A (SWI-like)

Subcellular Location

Nucleus

Protein Name

AT rich interactive domain 1A (SWI-like)

Isotype

IgG

Contents

ARID1A antibody is supplied in PBS containing 0.02% sodium azide.