Anti-FOXA1 Antibody

About this product

Boster Bio Anti-FOXA1 Antibody catalog # A00266. Tested in WB, ICC/IF, IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00266

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; Forkhead box protein A1; Transcription factor 3A; TCF-3A; FOXA1; HNF3A, TCF3A

Gene Name

FOXA1

UniProt

P55317

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein

Clonality

Polyclonal

Tissue Specificity

Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.

Applications

WB, ICC, IF, IHC

Purification

ProA affinity purified

Concentration

1 mg/mL

Form

Liquid

Function

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity) . Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity) . Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

49148 MW

Gene Name Synonym

Hepatocyte nuclear factor 3-alpha

Subcellular Location

Nucleus

Protein Name

Hepatocyte nuclear factor 3-alpha

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2