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Product details

Anti-DNA topoisomerase 1 TOP1 Antibody

Catalog number A00434

Price for A00434

Size / count 100 µL

About this product

Boster Bio Anti-DNA topoisomerase 1 TOP1 Antibody catalog # A00434. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number: A00434
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
Synonyms: DNA topoisomerase 1;5.99.1.2; DNA topoisomerase I; TOP1
Gene Name: TOP1
UniProt: P11387
Host: Rabbit
Reactivity: Human, Mouse
Immunogen: A synthetic peptide of human DNA topoisomerase I (DNA topoisomerase I (TOP1) ) (NP_003277.1) .
Clonality: Polyclonal
Tissue Specificity: Endothelial cells.
Applications: WB, IHC, IP
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Concentration: 1 mg/mL
Form: Liquid
Function: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (3'-phosphotyrosyl) -enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity) . Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
Storage Conditions: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight: 90726 MW
Gene Name Synonym: DNA topoisomerase 1
Subcellular Location: Nucleus, nucleolus
Protein Name: DNA topoisomerase 1
Contents: Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Frequently Asked Questions

What is the catalog number for Anti-DNA topoisomerase 1 TOP1 Antibody?

The catalog number for Anti-DNA topoisomerase 1 TOP1 Antibody is A00434.

What sizes are available for Anti-DNA topoisomerase 1 TOP1 Antibody?

Anti-DNA topoisomerase 1 TOP1 Antibody is available in the following size(s): 100 µL.

Is Anti-DNA topoisomerase 1 TOP1 Antibody in stock?

Anti-DNA topoisomerase 1 TOP1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Anti-DNA topoisomerase 1 TOP1 Antibody?

The safety data sheet for Anti-DNA topoisomerase 1 TOP1 Antibody is available here: https://www.bosterbio.com/msds?sku=A00434.

What is Anti-DNA topoisomerase 1 TOP1 Antibody used for?

Boster Bio Anti-DNA topoisomerase 1 TOP1 Antibody catalog # A00434. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.