Anti- (Mouse) Pdx1 Antibody (Center)

About this product

Boster Bio Anti- (Mouse) Pdx1 Antibody (Center) (Catalog # A00491-2) . Tested in WB, IF application (s) . This antibody reacts with Mouse.

Specifications

Catalog number

A00491-2-400ul

Size / count

400 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.

Synonyms

Pancreas/duodenum homeobox protein 1, Insulin promoter factor 1, IPF-1, Islet/duodenum homeobox 1, IDX-1, Somatostatin-transactivating factor 1, STF-1, Pdx1, Ipf1

Gene Name

Pdx1

UniProt

P52946

Host

Rabbit

Reactivity

Mouse

Immunogen

This Mouse Pdx1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 136-169 amino acids from the Central region of Mouse Pdx1.

Clonality

Polyclonal

Tissue Specificity

Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells)

Applications

WB, IF

Field of Research

Cancer, Cardiovascular, Developmental Biology, Signal Transduction, Stem Cells

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Observed Molecular Weight

30999 Da

Subcellular Location

Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:17052199}. Cytoplasm, cytosol

Isotype

Rabbit IgG

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.