Anti-Splicing factor 3B subunit 1 SF3B1 Antibody

About this product

Boster Bio Anti-Splicing factor 3B subunit 1 SF3B1 Antibody catalog # A00507. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00507

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Splicing factor 3B subunit 1; Pre-mRNA-splicing factor SF3b 155 kDa subunit; SF3b155; Spliceosome-associated protein 155; SAP 155; SF3B1; SAP155

Gene Name

SF3B1

UniProt

O75533

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 134-179 of Human SF3B1.

Clonality

Polyclonal

Applications

WB

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643) . SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937) . May also be involved in the assembly of the 'E' complex (PubMed:10882114) . Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

145830 MW

Applications Notes

WB: 1:500-1:1000

Gene Name Synonym

Splicing factor 3B subunit 1

Subcellular Location

Nucleus

Protein Name

Splicing factor 3B subunit 1

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2