Anti-ZEB1 Antibody

About this product

Boster Bio Anti-ZEB1 Antibody (Catalog # A00548) . Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human.

Specifications

Catalog number

A00548

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ZEB1, initially identified as the delta-crystallin enhancer binding protein delta EF1, is a DNA-binding protein that binds to a modified E-box sequence and has been implicated in postgastrulation embryogenesis. ZEB1 binds to the promoter of several hemapoietic genes, including interleukin-2, CD4, GATA-3, and alpha-integrin, and mice in which ZEB1 has been targeted show thymic atrophy, and severe defects in lymphocyte differentiation. Recent evidence suggests that ZEB1 also regulates the accumulation of adipose tissue and may play a role in obesity. Mutations in this gene have been associated with late-onset Fuchs endothelial corneal dystrophy.

Synonyms

BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, DELTAEF1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, TCF-8

Gene Name

ZEB1

UniProt

P37275

Host

Rabbit

Reactivity

Human

Cross Reactivity

Multiple isoforms of ZEB1 are known to exist.

Immunogen

ZEB1 antibody was raised against a 15 amino acid synthetic peptide near the center of human ZEB1. The immunogen is located within amino acids 530 - 580 of ZEB1.

Clonality

Polyclonal

Tissue Specificity

Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level) . Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.

Applications

ELISA, WB, IHC-P, ICC, IF

Field of Research

Stem Cells

Purification

ZEB1 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3') . Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.

Storage Conditions

ZEB1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

124074 MW

Gene Name Synonym

Zinc finger E-box binding homeobox 1

Subcellular Location

Nucleus

Protein Name

Zinc finger E-box binding homeobox 1

Isotype

IgG

Contents

ZEB1 Antibody is supplied in PBS containing 0.02% sodium azide.