Anti-LEF-1 (phospho-S42) Antibody

About this product

Boster Bio Anti-LEF-1 (phospho-S42) Antibody catalog # A00605S42. Tested in WB applications. This antibody reacts with Human, Rat.

Specifications

Catalog number

A00605S42

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha; LEF1

Gene Name

LEF1

UniProt

Q9UJU2

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Synthetic phosphopeptide derived from human LEF-1 around the phosphorylation site of Serine 42

Clonality

Polyclonal

Tissue Specificity

Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.

Applications

WB

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity) . Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1- independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

44201 MW

Applications Notes

WB: 1:500-1:1000

Gene Name Synonym

Lymphoid enhancer-binding factor 1

Subcellular Location

Nucleus

Protein Name

Lymphoid enhancer-binding factor 1

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2