Anti-DNA-PK (phospho-S2056) PRKDC Antibody

About this product

Boster Bio Anti-DNA-PK (phospho-S2056) PRKDC Antibody catalog # A00645S2056. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00645S2056

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs;2.7.11.1; DNPK1; p460; PRKDC; HYRC, HYRC1

Gene Name

PRKDC

UniProt

P78527

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S2056 of human DNA-PKcs (NP_008835.5) .

Clonality

Polyclonal

Applications

WB, ICC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V (D) J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V (D) J recombination by activation of the hairpin endonuclease artemis (DCLRE1C) . The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect mechanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation. Plays a role in the regulation of DNA virus- mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

469089 MW

Gene Name Synonym

DNA-dependent protein kinase catalytic subunit

Subcellular Location

Nucleus

Protein Name

DNA-dependent protein kinase catalytic subunit

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2