Anti-PKA alpha/ beta/ gamma cat PRKACA Antibody

About this product

Boster Bio Anti-PKA alpha/ beta/ gamma cat PRKACA Antibody catalog # A00653. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Pig.

Specifications

Catalog number

A00653

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

CAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha;2.7.11.11; PRKACA; PKACA

Gene Name

PRKACA

UniProt

P17612

Host

Rabbit

Reactivity

Human, Mouse, Rat, Pig

Immunogen

The antiserum was produced against synthesized peptide derived from human PKA alpha/beta CAT. AA range:166-215

Clonality

Polyclonal

Tissue Specificity

Isoform 1 is ubiquitous. Isoform 2 is sperm- specific and is enriched in pachytene spermatocytes but is not detected in round spermatids. .

Applications

WB, IHC, IF, ELISA

Purification

Immunogen affinity purified

Concentration

1 mg/mL

Form

Liquid

Function

Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose- mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B- alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha- difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca (2+), leading to reduced amplitude and increased frequency of store overload-induced Ca (2+) release (SOICR) characterized by an increased rate of Ca (2+) release and propagation velocity of spontaneous Ca (2+) waves, despite reduced wave amplitude and resting cytosolic Ca (2+) . PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT) . Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

40590 MW

Gene Name Synonym

CAMP-dependent protein kinase catalytic subunit alpha

Subcellular Location

Cytoplasm. Cell membrane. Nucleus . Mitochondrion . Membrane ; Lipid-anchor . Translocates into the nucleus (monomeric catalytic subunit) . The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity) . .

Isotype

IgG

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.