Anti-ERCC2 Antibody

About this product

Boster Bio Anti-ERCC2 Antibody catalog # A00694. Tested in WB, ICC/IF applications. This antibody reacts with Human, Rat.

Specifications

Catalog number

A00694

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

General transcription and DNA repair factor IIH helicase subunit XPD; TFIIH subunit XPD;3.6.4.12; Basic transcription factor 2 80 kDa subunit; BTF2 p80; CXPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; TFIIH basal transcription factor complex 80 kDa subunit; TFIIH 80 kDa subunit; TFIIH p80; Xeroderma pigmentosum group D-complementing protein; ERCC2; XPD, XPDC

Gene Name

ERCC2

UniProt

P18074

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein of human XPD/XPD/ERCC2 (NP_001124339.1) .

Clonality

Polyclonal

Applications

WB, ICC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre- initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

86909 MW

Gene Name Synonym

General transcription and DNA repair factor IIH helicase subunit XPD

Subcellular Location

Nucleus

Protein Name

General transcription and DNA repair factor IIH helicase subunit XPD

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2