Anti-RICK RIPK2 Antibody

About this product

Boster Bio Anti-RICK RIPK2 Antibody (Catalog # A00818) . Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00818

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively. RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-κB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1β . The messenger RNA of RICK is expressed in multiple human tissues.

Synonyms

CCK, RICK, RIP2, CARD3, GIG30, CARDIAK, UNQ277/PRO314/PRO34092, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD-containing IL-1 beta ICE-kinase

Gene Name

RIPK2

UniProt

O43353

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

RICK antibody was raised against a peptide corresponding to 20 amino acids near the amino terminus of human RICK. The immunogen is located within the first 50 amino acids of RICK.

Clonality

Polyclonal

Tissue Specificity

Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.

Applications

ELISA, WB, ICC, IF

Field of Research

Apoptosis

Purification

RICK Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Storage Conditions

RICK antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

61195 MW

Gene Name Synonym

Receptor-interacting serine-threonine kinase 2

Subcellular Location

Cytoplasm .

Prediction Reactivity

Bovine

Protein Name

Receptor-interacting serine-threonine kinase 2

Isotype

IgG

Contents

RICK Antibody is supplied in PBS containing 0.02% sodium azide.