Anti-LGI1 Antibody

About this product

Boster Bio Anti-LGI1 Antibody (Catalog # A00850) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00850

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist; this LGI1 antibody will recognize only the longer form.

Synonyms

EPT, ETL1, ADLTE, ADPAEF, ADPEAF, IB1099, EPITEMPIN, EPT, UNQ775/PRO1569, Leucine-rich glioma-inactivated protein 1, Epitempin-1

Gene Name

LGI1

UniProt

O95970

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

This LGI1 antibody is predicted to be specific to LGI1 and not recognize other LGI proteins.

Immunogen

LGI1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human LGI1. The immunogen is located within amino acids 370 - 420 of LGI1.

Clonality

Polyclonal

Tissue Specificity

Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level) . Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level) .

Applications

ELISA, WB, IHC-P, IF

Field of Research

Neuroscience

Purification

LGI1 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity) . Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.

Storage Conditions

LGI1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

63818 MW

Gene Name Synonym

Leucine-rich, glioma inactivated 1

Subcellular Location

Secreted

Prediction Reactivity

Bovine

Protein Name

Leucine-rich, glioma inactivated 1

Isotype

IgG

Contents

LGI1 Antibody is supplied in PBS containing 0.02% sodium azide.