- Catalog number
- A00881
- Size / count
- 100 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Synonyms
- Histone-lysine N-methyltransferase SETDB1;2.1.1.43; ERG-associated protein with SET domain; ESET; Histone H3-K9 methyltransferase 4; H3-K9-HMTase 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1; SETDB1; KIAA0067, KMT1E
- Gene Name
- SETDB1
- UniProt
- Q15047
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Immunogen
- Recombinant full length Human SETDB1.
- Clonality
- Polyclonal
- Tissue Specificity
- Widely expressed. High expression in testis.
- Applications
- WB, IHC, IF
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
- Concentration
- 1 mg/mL
- Form
- Liquid
- Function
- Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306) . Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306) . Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306) . The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610) .
- Storage Conditions
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
- Observed Molecular Weight
- 143157 MW
- Gene Name Synonym
- Histone-lysine N-methyltransferase SETDB1
- Subcellular Location
- Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
- Protein Name
- Histone-lysine N-methyltransferase SETDB1
- Contents
- Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
