Anti-ATF3 Antibody

About this product

Boster Bio Anti-ATF3 Antibody (Catalog # A00904) . Tested in ELISA, WB applications. This antibody reacts with Human.

Specifications

Catalog number

A00904

Size / count

100 µg

Dry ice

Yes

Hazardous

No

Discontinued

No

Background

ATF3, or Activating Transcription Factor 3, is a member of mammalian activation TF/CREB protein family of transcription factors. ATF3 binds the cAMP response element (cre) (consensus: 5'-gtgacgt[ac][ag]-3'), a sequence present in many viral and cellular promoters. However, ATF3 represses rather than activates transcription from promoters with ATF sites stabilizing inhibitory co-factors at the promoter. Alternate splicing forms of ATF3, called ATF3 delta Zip, lack the leucine zipper domain and do not bind DNA. ATF3 delta Zip stimulates transcription, presumably by sequestering inhibitory co-factors away from the promoter. Human ATF3 (SwissProt 18847) is a 20575 Da protein composed of 181 amino acids.

Synonyms

ATF3, ATF 3, Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3, Activating transcription factor 3

Gene Name

ATF3

UniProt

P18847

Host

Rabbit

Reactivity

Human

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATF3.

Clonality

Polyclonal

Applications

ELISA, WB

Field of Research

Cancer, Epigenetics and Nuclear Signaling, Transcription, Tumor Biomarkers

Purification

This affinity-purified antibody is directed against human ATF3 and is useful in determining its presence in various assays including ELISA and western blotting. This polyclonal antibody recognizes over-expressed ATF3 protein found in various expression systems. Reactivity is observed against human ATF3. Cross-reactivity with ATF3 from other mammalian sources has not been tested.

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

20576 MW

Gene Name Synonym

Cyclic AMP-dependent transcription factor ATF-3

Subcellular Location

Nucleus.

Isotype

IgG

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide