Anti-ATF2 (phospho-T69) Antibody

About this product

Boster Bio Anti-ATF2 (phospho-T69) Antibody catalog # A00916T69. Tested in IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00916T69

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2;2.3.1.48

Gene Name

ATF2

UniProt

P15336

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human ATF2 around the phosphorylation site of Threonine 69.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed, with more abundant expression in the brain.

Applications

IHC, IP

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Transcriptional activator which regulates the transcription of various genes, including those involved in anti- apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3') . In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR) -induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF) . Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

54537 MW

Gene Name Synonym

Cyclic AMP-dependent transcription factor ATF-2

Subcellular Location

Nucleus. Cytoplasm. Mitochondrion outer membrane. Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-52 is required for its nuclear localization and negatively regulates its mitochondrial localization. Co- localizes with the MRN complex in the IR-induced foci (IRIF) .

Protein Name

Cyclic AMP-dependent transcription factor ATF-2

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2