Anti-Axin-1 AXIN1 Antibody

About this product

Boster Bio Anti-Axin-1 AXIN1 Antibody (Catalog # A00986) . Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00986

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), beta-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of beta-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor.

Synonyms

AXIN, PPP1R49, AXIN, Axin-1, Axis inhibition protein 1, hAxin

Gene Name

AXIN1

UniProt

O15169

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

AXIN1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human AXIN1. The immunogen is located within amino acids 620 - 670 of AXIN1.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

ELISA, WB, IF

Field of Research

Signal Transduction

Purification

AXIN1 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed:12192039, PubMed:27098453) . Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (PubMed:12192039) . In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B (PubMed:12192039) . Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (PubMed:16601693) . Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development (PubMed:17210684) . Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (PubMed:17210684) .

Storage Conditions

AXIN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

95635 MW

Gene Name Synonym

Axin 1

Subcellular Location

Cytoplasm

Prediction Reactivity

Chicken

Protein Name

Axin 1

Isotype

IgG

Contents

AXIN1 Antibody is supplied in PBS containing 0.02% sodium azide.