Anti-Tyrosine-protein kinase HCK Hck Antibody

About this product

Boster Bio Anti-Tyrosine-protein kinase HCK Hck Antibody catalog # A01073. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A01073

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Tyrosine-protein kinase HCK;2.7.10.2; Hematopoietic cell kinase; Hemopoietic cell kinase; p59-HCK/p60-HCK; p59Hck; p61Hck; HCK

Gene Name

HCK

UniProt

P08631

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human HCK. AA range:381-430

Tissue Specificity

Detected in monocytes and neutrophils (at protein level) . Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil. .

Applications

WB, IHC, IF, ELISA

Purification

Immunogen affinity purified

Concentration

1 mg/mL

Form

Liquid

Function

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

59600 MW

Gene Name Synonym

Tyrosine-protein kinase HCK

Subcellular Location

Isoform 1: Lysosome. Membrane; Lipid-anchor. Cell projection, podosome membrane; Lipid-anchor. Cytoplasm, cytosol. Associated with specialized secretory lysosomes called azurophil granules. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated.

Isotype

IgG

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.