Anti-UHRF1 Antibody (ChIP Grade)

About this product

Boster Bio Anti-UHRF1 Antibody (ChIP Grade) catalog # A01156. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A01156

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

E3 ubiquitin-protein ligase UHRF1;2.3.2.27; Inverted CCAAT box-binding protein of 90 kDa; Nuclear protein 95; Nuclear zinc finger protein Np95; HuNp95; hNp95; RING finger protein 106; RING-type E3 ubiquitin transferase UHRF1; Transcription factor ICBP90; Ubiquitin-like PHD and RING finger domain-containing protein 1; hUHRF1; Ubiquitin-like-containing PHD and RING finger domains protein 1; UHRF1; ICBP90, NP95, RNF106

Gene Name

UHRF1

UniProt

Q96T88

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein of human UHRF1 (NP_001041666.1) .

Clonality

Polyclonal

Tissue Specificity

Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.

Applications

WB, IHC, ICC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

89814 MW

Gene Name Synonym

E3 ubiquitin-protein ligase UHRF1

Subcellular Location

Nucleus

Protein Name

E3 ubiquitin-protein ligase UHRF1

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2