Anti-eIF-2-alpha kinase GCN2 EIF2AK4 Antibody

About this product

Boster Bio Anti-eIF-2-alpha kinase GCN2 EIF2AK4 Antibody catalog # A01172. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A01172

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

EIF-2-alpha kinase GCN2

Gene Name

EIF2AK4

UniProt

Q9P2K8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human GCN2 (NP_001013725.2) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed (PubMed:10504407) . Expressed in lung, smooth muscle cells and macrophages (PubMed:24292273) .

Applications

WB, IHC, ICC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' in response to low amino acid availability (PubMed:25329545) . Plays a role as an activator of the integrated stress response (ISR) required for adapatation to amino acid starvation. Converts phosphorylated eIF- 2-alpha/EIF2S1 either to a competitive inhibitor of the translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs (By similarity) . Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:26102367) . Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory. Plays a role in neurite outgrowth inhibition. Plays a proapoptotic role in response to glucose deprivation. Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress- activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity) . Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

186911 MW

Gene Name Synonym

EIF-2-alpha kinase GCN2

Subcellular Location

Cytoplasm

Protein Name

EIF-2-alpha kinase GCN2

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2