Anti-SHANK3 Antibody

About this product

Boster Bio Anti-SHANK3 Antibody (Catalog # A01231) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A01231

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SH3 and multiple ankyrin repeat domains 3 (SHANK3), a member of the Shank gene family, plays a role in synapse formation and dendritic spine maturation. Shank proteins (Shank 1-3) containing PDZ domains are scaffold proteins of the postsynaptic density (PSD) that connect neurotransmitter receptors and ion channels proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Mutations of SHANK3 are a cause of autism spectrum disorder (ASD) and the neurological symptoms of 22q13.3 deletion syndrome.

Synonyms

PSAP2, SCZD15, PROSAP2, SPANK-2, DEL22q13.3, KIAA1650, PSAP2, SH3 and multiple ankyrin repeat domains protein 3, Proline-rich synapse-associated protein 2, Shank3

Gene Name

SHANK3

UniProt

Q9BYB0

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

At least three alternatively spliced transcript isoforms of SHANK3 are known to exist.

Immunogen

SHANK3 antibody was raised against a 19 amino acid synthetic peptide near the center of human SHANK3. The immunogen is located within amino acids 890 - 940 of SHANK3.

Clonality

Polyclonal

Tissue Specificity

Expressed in the cerebral cortex and the cerebellum.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Neuroscience

Purification

SHANK3 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor- mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.

Storage Conditions

SHANK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

184667 MW

Gene Name Synonym

SH3 and multiple ankyrin repeat domains 3

Subcellular Location

Cytoplasm

Protein Name

SH3 and multiple ankyrin repeat domains 3

Isotype

IgG

Contents

SHANK3 Antibody is supplied in PBS containing 0.02% sodium azide.