Anti-STUB1 Antibody

About this product

Boster Bio Anti-STUB1 Antibody catalog # A01236. Tested in WB, ICC, IF, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A01236

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

E3 ubiquitin-protein ligase CHIP ;2.3.2.27

Gene Name

STUB1

UniProt

Q9UNE7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human STUB1 aa 1-200.

Clonality

Polyclonal

Tissue Specificity

Expressed in differentiated myotubes (at protein level) (PubMed:17369820) . Highly expressed in skeletal muscle, heart, pancreas, brain and placenta (PubMed:10330192, PubMed:11435423) . Detected in kidney, liver and lung (PubMed:10330192, PubMed:11435423) .

Applications

WB, ICC, IF, IHC, IP, Flow Cytometry

Purification

ProA affinity purified

Concentration

1 mg/mL

Form

Liquid

Function

E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF- BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Acts as a co-chaperone for HSPA1A and HSPA1B chaperone proteins and promotes ubiquitin- mediated protein degradation (PubMed:27708256) . Negatively regulates the suppressive function of regulatory T-cells (Treg) during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner (PubMed:23973223) . Likely mediates polyubiquitination and downregulates plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. Negatively regulates TGF-beta signaling by modulating the basal level of SMAD3 via ubiquitin-mediated degradation (PubMed:24613385) . May regulate myosin assembly in striated muscles together with UBE4B and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed:17369820) . Mediates ubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation (PubMed:29883609) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

34856 MW

Gene Name Synonym

E3 ubiquitin-protein ligase CHIP

Subcellular Location

Cytoplasm

Protein Name

E3 ubiquitin-protein ligase CHIP

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2