- Catalog number
- A01295
- Size / count
- 0.1 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.
- Synonyms
- CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, CASH, CASP8 and FADD-like apoptosis regulator, Caspase homolog
- Gene Name
- CFLAR
- UniProt
- O15519
- Host
- Rabbit
- Reactivity
- Mouse
- Cross Reactivity
- FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.
- Immunogen
- FLIP antibody was raised against a 17 amino acid peptide near the carboxy terminus of mouse FLIP. The immunogen is located within the last 50 amino acids of FLIP.
- Clonality
- Polyclonal
- Tissue Specificity
- Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
- Applications
- ELISA, WB, ICC
- Field of Research
- Apoptosis
- Purification
- FLIP Antibody is Antibody is DEAE purified.
- Concentration
- 1 mg/mL
- Form
- Liquid
- Function
- Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
- Storage Conditions
- FLIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Observed Molecular Weight
- 55344 MW
- Gene Name Synonym
- CASP8 and FADD-like apoptosis regulator
- Subcellular Location
- Cytoplasm.
- Prediction Reactivity
- Human
- Protein Name
- CASP8 and FADD-like apoptosis regulator
- Isotype
- IgG
- Contents
- FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
