- Catalog number
- A01337
- Size / count
- 100 µg
- Dry ice
- Yes
- Hazardous
- No
- Discontinued
- No
- Background
- The NCOA3 (also known as nuclear receptor coactivator 3 isoform a) is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions in a hormone-dependent fashion. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. NCOA3 probably acts via remodeling of chromatin and is involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs) . NCOA3 is also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. NCOA3 may be associated with diseases such as breast cancer and meningothelial meningioma. Anti-NCOA3 Antibody is useful for researchers interested in cancer research, chromatin binding, transcription coactivator activity, Immunology, and Nuclear Signaling research.
- Synonyms
- NCOA3, NCOA 3, NCOA-3, Nuclear receptor coactivator 3 NCoA-3, Thyroid hormone receptor activator molecule 1, TRAM-1, pCIP, bHLHe42, Receptor-associated coactivator 3, RAC-3, Amplified in breast cancer 1 protein, AIB-1, Steroid receptor coactivator protein 3, SRC-3, ACTR, AIB1, BHLHE42, RAC3, TRAM1
- Gene Name
- NCOA3
- UniProt
- Q9Y6Q9
- Host
- Rabbit
- Reactivity
- Human
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human NCOA3 isoform a protein.
- Clonality
- Polyclonal
- Tissue Specificity
- Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
- Applications
- ELISA, IHC, IP, WB
- Field of Research
- Acetylation, Cancer, Cancer Metabolism,2339, Chromatin Modifying Enzymes, Co-Activators/Co-Repressors, Epigenetics and Nuclear Signaling, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Obesity, Pathways and Processes, Signal Transduction, Signaling Pathway
- Purification
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with several isoforms of human NCOA3 protein (a, b, d, e and f) . A BLAST analysis was used to suggest cross-reactivity with NCOA3 from human and macaque sources based on a 100% homology with the immunizing sequence. Expect partial reactivity with NCOA3 from dog, bovine, mouse and rat sources based on partial sequence homology. Cross-reactivity with NCOA3 from other sources has not been determined.
- Concentration
- 1.24 mg/mL by UV absorbance at 280 nm
- Form
- Liquid (sterile filtered)
- Function
- Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs) . Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
- Shipping Conditions
- Dry Ice Shipping
- Storage Conditions
- Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- Observed Molecular Weight
- 155293 MW
- Gene Name Synonym
- Nuclear receptor coactivator 3
- Subcellular Location
- Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
- Isotype
- IgG
- Contents
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
