Anti-Polycomb protein EED EED Antibody

About this product

Boster Bio Anti-Polycomb protein EED EED Antibody (Catalog # A01345) . Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A01345

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The EED protein (WAIT-1 or WD protein associated with integrin cytoplasmic tails-1), also called embryonic ectoderm development, is a member of the superfamily of WD-40 repeat proteins and widely conserved polycomb group (PcG) family of proteins. The polycomb group (PcG) is a large and evolutionarily conserved set of genes whose products act in multimeric complexes to modify histones, which are then thought to cause stable and heritable states of transcriptional repression. EED shuttles between the nucleus and the plasma membrane and can interact with the cytoplasmic tail of integrin β7 subunit. EED exerted an antiviral activity at the late stage of HIV-1 replication.

Synonyms

HEED, WAIT1, Polycomb protein EED, WD protein associating with integrin cytoplasmic tails 1, hEED

Gene Name

EED

UniProt

O75530

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

Four alternatively spliced transcript variants have been observed.

Immunogen

EED antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EED. The immunogen is located within amino acids 30 - 80 of EED.

Clonality

Polyclonal

Tissue Specificity

Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.

Applications

ELISA, WB, IF

Field of Research

Stem Cells

Purification

EED Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Storage Conditions

EED antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

50198 MW

Gene Name Synonym

Embryonic ectoderm development

Subcellular Location

Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.

Prediction Reactivity

Chicken, Bovine

Protein Name

Embryonic ectoderm development

Isotype

IgG

Contents

EED Antibody is supplied in PBS containing 0.02% sodium azide.