Anti-DNA pol iota Antibody

About this product

Boster Bio Anti-DNA pol iota Antibody catalog # A01376. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A01376

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

DNA polymerase iota;2.7.7.7; Eta2; RAD30 homolog B; POLI; RAD30B

Gene Name

POLI

UniProt

Q9UNA4

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human POLI. AA range:641-690

Tissue Specificity

Ubiquitous. Highly expressed in testis. .

Applications

WB, ELISA

Purification

Immunogen affinity purified

Concentration

1 mg/mL

Form

Liquid

Function

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

83006 MW

Gene Name Synonym

DNA polymerase iota

Subcellular Location

Nucleus . Accumulates at replication forks after DNA damage.

Isotype

IgG

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.