Anti-PRMT1 Monoclonal Antibody

About this product

Boster Bio Anti-PRMT1 Monoclonal Antibody catalog # A01417. Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Dog, Rabbit.

Specifications

Catalog number

A01417

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Protein arginine N-methyltransferase 1;2.1.1.319 ; Histone-arginine N-methyltransferase PRMT1; Interferon receptor 1-bound protein 4; PRMT1; HMT2, HRMT1L2, IR1B4

Gene Name

PRMT1

UniProt

Q99873

Host

Mouse

Reactivity

Human, Mouse, Rat, Dog, Rabbit

Immunogen

Purified recombinant human PRMT1 protein fragments expressed in E.coli.

Clonality

Monoclonal

Tissue Specificity

Widely expressed. .

Applications

WB

Purification

Protein A purified

Concentration

1 mg/mL

Form

Liquid

Function

Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER) -mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC) -binding activity (PubMed:25284789) . Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

41516 MW

Gene Name Synonym

Protein arginine N-methyltransferase 1

Subcellular Location

Nucleus . Nucleus, nucleoplasm . Cytoplasm, cytosol . Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction (By similarity) . .

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.