Anti-PAK gamma PAK2 Antibody

About this product

Boster Bio Anti-PAK gamma PAK2 Antibody catalog # A01419. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A01419

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Serine/threonine-protein kinase PAK 2;2.7.11.1; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58; PAK-2p27; p27; PAK-2p34; p34; C-t-PAK2; PAK2

Gene Name

PAK2

UniProt

Q13177

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human PAK2. AA range:5-54

Tissue Specificity

Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.

Applications

WB, IHC, IF, ELISA

Purification

Immunogen affinity purified

Concentration

1 mg/mL

Form

Liquid

Function

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF- induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase- activated PAK2 phosphorylates MKNK1 and reduces cellular translation. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

58043 MW

Gene Name Synonym

Serine/threonine-protein kinase PAK 2

Subcellular Location

Serine/threonine-protein kinase PAK 2: Cytoplasm. MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane.

Isotype

IgG

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.