Anti-PARN Antibody

About this product

Boster Bio Anti-PARN Antibody catalog # A01501. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Specifications

Catalog number

A01501

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Poly (A) -specific ribonuclease PARN;3.1.13.4; Deadenylating nuclease; Deadenylation nuclease; Polyadenylate-specific ribonuclease; PARN; DAN

Gene Name

PARN

UniProt

O95453

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant fusion protein of human PARN (NP_002573.1) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

WB, ICC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

3'-exoribonuclease that has a preference for poly (A) tails of mRNAs, thereby efficiently degrading poly (A) tails. Exonucleolytic degradation of the poly (A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly (A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly (A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly (A) tails of AREs mRNAs, which constitutes the first step of destabilization (PubMed:10882133, PubMed:11359775, PubMed:12748283, PubMed:15175153, PubMed:9736620) . Also able to recognize and trim poly (A) tails of microRNAs such as MIR21 and H/ACA box snoRNAs (small nucleolar RNAs) leading to microRNAs degradation or snoRNA increased stability (PubMed:25049417, PubMed:22442037) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

73451 MW

Gene Name Synonym

Poly (A) -specific ribonuclease PARN

Subcellular Location

Nucleus

Protein Name

Poly (A) -specific ribonuclease PARN

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2