Anti-Cot (phospho-T290) MAP3K8 Antibody

About this product

Boster Bio Anti-Cot (phospho-T290) MAP3K8 Antibody catalog # A01696T290. Tested in IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A01696T290

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Mitogen-activated protein kinase kinase kinase 8;2.7.11.25; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2; TPL-2; MAP3K8; COT, ESTF

Gene Name

MAP3K8

UniProt

P41279

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Cot around the phosphorylation site of Threonine 290.

Clonality

Polyclonal

Tissue Specificity

Expressed in several normal tissues and human tumor-derived cell lines.

Applications

IHC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Required for lipopolysaccharide (LPS) -induced, TLR4- mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF- alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B- cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

52925 MW

Gene Name Synonym

Mitogen-activated protein kinase kinase kinase 8

Subcellular Location

Cytoplasm

Protein Name

Mitogen-activated protein kinase kinase kinase 8

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2