Anti-PAK4/5/6 (phospho-S474) Antibody

About this product

Boster Bio Anti-PAK4/5/6 (phospho-S474) Antibody catalog # A01723S474. Tested in WB, IHC, ICC applications. This antibody reacts with Human.

Specifications

Catalog number

A01723S474

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Serine/threonine-protein kinase PAK 4;2.7.11.1; p21-activated kinase 4; PAK-4; PAK4; KIAA1142

Gene Name

PAK4

UniProt

O96013/Q9NQU5/Q9P286

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Serine 474.

Clonality

Polyclonal

Tissue Specificity

Highest expression in prostate, testis and colon.

Applications

WB, IHC, ICC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

64072 MW

Gene Name Synonym

Serine/threonine-protein kinase PAK 4

Subcellular Location

Cytoplasm

Protein Name

Serine/threonine-protein kinase PAK 4

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2