Anti-DIS3 Antibody

About this product

Boster Bio Anti-DIS3 Antibody (Catalog # A01736) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A01736

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The exosome is involved in a multitude of cellular RNA processing and degradation events. DIS3, also known as exosome complex exonuclease RRP44, is a ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. DIS3 localizes to both the cytoplasm and the nucleus and contains one PINc domain. It is widely expressed with highest expression in testis and is required for processing of 7S pre-RNA into a mature nuclear complex and, ultimately, for proper mitotic progression. Abnormal expression levels of DIS3 may be associated with colon cancer, suggesting a role for DIS3 in tumorigenesis.

Synonyms

RRP44, dis3p, EXOSC11, KIAA1008, 2810028N01Rik, RRP44, Exosome complex exonuclease RRP44, Protein DIS3 homolog

Gene Name

DIS3

UniProt

Q9Y2L1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

Multiple isoforms of DIS3 are known to exist.

Immunogen

DIS3 antibody was raised against an 18 amino acid peptide near the amino terminus of human DIS3. The immunogen is located within amino acids 90 - 140 of DIS3.

Clonality

Polyclonal

Tissue Specificity

Widely expressed.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Cancer, Cell Cycle

Purification

DIS3 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.

Storage Conditions

DIS3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Observed Molecular Weight

109003 MW

Gene Name Synonym

DIS3 mitotic control homolog (S. cerevisiae)

Subcellular Location

Cytoplasm

Protein Name

DIS3 mitotic control homolog (S. cerevisiae)

Isotype

IgG

Contents

DIS3 Antibody is supplied in PBS containing 0.02% sodium azide.