Anti-Cleaved-COL4A3 (L1425) Antibody

About this product

Boster Bio Anti-Cleaved-COL4A3 (L1425) Antibody catalog # A01755. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A01755

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Collagen alpha-3 (IV) chain; Goodpasture antigen; Tumstatin; COL4A3

Gene Name

COL4A3

UniProt

Q01955

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Collagen IV alpha3. AA range:1376-1425

Clonality

Polyclonal

Tissue Specificity

Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. According to PubMed:8083201, alpha 3 type IV collagen is not detected in heart, brain, placenta, liver, pancreas, extrasynaptic muscle fibers, endoneurial and perineurial nerves, fetal brain, fetal heart and fetal liver. According to PubMed:7523402, alpha 3 type IV collagen is strongly expressed in pancreas, neuroretina and calvaria and not expressed in adrenal, ileum and skin. Isoform 1 and isoform 3 are strongly expressed in kidney, lung, suprarenal capsule, muscle and spleen, in each of these tissues isoform 1 is more abundant than isoform 3. Isoform 1 and isoform 3 are expressed at low levels in artery, fat, pericardium and peripherical nerve, but not in placenta, mesangium, skin, pleura and cultured umbilical endothelial cells.

Applications

WB, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/mL

Form

Liquid

Function

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

161813 MW

Gene Name Synonym

Collagen alpha-3 (IV) chain

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane. Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement membrane (TBM) and synaptic basal lamina (BL) . .

Protein Name

Collagen alpha-3 (IV) chain

Isotype

IgG

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.