- Catalog number
- A01768
- Size / count
- 0.1 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.
- Synonyms
- APG3, APG3L, PC3-96, APG3-LIKE, APG3, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, APG3-like
- Gene Name
- ATG3
- UniProt
- Q9NT62
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Immunogen
- ATG3 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG3. The immunogen is located within amino acids 180 - 230 of ATG3.
- Clonality
- Polyclonal
- Tissue Specificity
- Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
- Applications
- ELISA, WB, IHC-P, IF
- Field of Research
- Autophagy
- Purification
- ATG3 Antibody is affinity chromatography purified via peptide column.
- Concentration
- 1 mg/mL
- Form
- Liquid
- Function
- E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) . The ATG12- ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE) . This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt) . Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
- Storage Conditions
- ATG3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Observed Molecular Weight
- 35864 MW
- Gene Name Synonym
- ATG3 autophagy related 3 homolog (S. cerevisiae)
- Subcellular Location
- Cytoplasm
- Prediction Reactivity
- Bovine
- Protein Name
- ATG3 autophagy related 3 homolog (S. cerevisiae)
- Isotype
- IgG
- Contents
- ATG3 Antibody is supplied in PBS containing 0.02% sodium azide.
