Anti-Importin subunit alpha-1 KPNA2 Antibody

About this product

Boster Bio Anti-Importin subunit alpha-1 KPNA2 Antibody (Catalog # A01776) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A01776

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA2 is the alpha subunit 2 of karyopherin, which forms a complex with importin subunit beta-1 and functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. It is ubiquitously expressed and contains an IBB/importin beta domain, ten Armadillo repeats that bind "cargo" and three intervening nuclear localization sequences (NLSs) . It has recently been reported to play an important role in tumorigenesis and tumor progression.

Synonyms

QIP2, RCH1, IPOA1, SRP1alpha, SRP1, Importin subunit alpha-1, Karyopherin subunit alpha-2

Gene Name

KPNA2

UniProt

P52292

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

KPNA2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA2. The immunogen is located within amino acids 40 - 90 of KPNA2.

Clonality

Polyclonal

Tissue Specificity

Expressed ubiquitously.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Cancer, Cell Cycle

Purification

KPNA2 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Storage Conditions

KPNA2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

57862 MW

Gene Name Synonym

Karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

Subcellular Location

Cytoplasm

Protein Name

Karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

Isotype

IgG

Contents

KPNA2 Antibody is supplied in PBS containing 0.02% sodium azide.