- Catalog number
- A01804
- Size / count
- 100 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Synonyms
- Tyrosyl-DNA phosphodiesterase 2; Tyr-DNA phosphodiesterase 2; hTDP2;3.1.4.-;5'-tyrosyl-DNA phosphodiesterase;5'-Tyr-DNA phosphodiesterase; ETS1-associated protein 2; ETS1-associated protein II; EAPII; TRAF and TNF receptor-associated protein; Tyrosyl-RNA phosphodiesterase; VPg unlinkase; TDP2; EAP2, TTRAP; AD-022
- Gene Name
- TDP2
- UniProt
- O95551
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Immunogen
- Recombinant fusion protein of human TDP2 (NP_057698.2) .
- Clonality
- Polyclonal
- Tissue Specificity
- Widely expressed (PubMed:10764746) . Highly expressed in various brain regions, including the frontal and occipital lobes, the hippocampus, the striatum and the cerebellum (PubMed:24658003) .
- Applications
- WB
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
- Concentration
- 1 mg/mL
- Form
- Liquid
- Function
- DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Acts as a regulator of ribosome biogenesis following stress. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'- tyrosyl-DNA phosphodiesterase in cells. Also acts as an adapter by participating in the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF-beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non- canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation.
- Storage Conditions
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
- Observed Molecular Weight
- 40930 MW
- Applications Notes
- WB: 1:500-1:2000
- Gene Name Synonym
- Tyrosyl-DNA phosphodiesterase 2
- Subcellular Location
- Nucleus. Nucleus, PML body. Nucleus, nucleolus. Cytoplasm. Localizes to nucleolar cavities following stress; localization to nucleolus is dependent on PML protein.
- Protein Name
- Tyrosyl-DNA phosphodiesterase 2
- Contents
- Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
