Anti-Forkhead box protein O4 FOXO4 Antibody

About this product

Boster Bio Anti-Forkhead box protein O4 FOXO4 Antibody (Catalog # A01819) . Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A01819

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

FOXO4 is a ubiquitously expressed protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In the absence of PI3K/AKT activation, FOXO4 localizes in the nucleus where it functions as a transcription factor. FOXO4 can also be phosphorylated by JNK following induction of reactive oxygen species (ROS), resulting in transcriptional activation and the induction of a negative feedback mechanism to counteract the ROS. It is through this mechanism that FOXO4 is thought to sensitize cancer cells to doxorubicin-mediated toxicity.

Synonyms

AFX, AFX1, MLLT7, AFX, Forkhead box protein O4, Fork head domain transcription factor AFX1

Gene Name

FOXO4

UniProt

P98177

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

FOXO4 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human FOXO4. The immunogen is located within amino acids 30 - 80 of FOXO4.

Clonality

Polyclonal

Tissue Specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

Applications

ELISA, WB, ICC, IF

Field of Research

Cancer, Cell Cycle

Purification

FOXO4 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

Storage Conditions

FOXO4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

53684 MW

Gene Name Synonym

Forkhead box O4

Subcellular Location

Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.

Protein Name

Forkhead box O4

Isotype

IgG

Contents

FOXO4 Antibody is supplied in PBS containing 0.02% sodium azide.