Anti-Rad51C (E185) Antibody

About this product

Boster Bio Anti-Rad51C (E185) Antibody catalog # A01837. Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A01837

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

DNA repair protein RAD51 homolog 3; R51H3; RAD51 homolog C; RAD51-like protein 2; RAD51C; RAD51L2

Gene Name

RAD51C

UniProt

O43502

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 161-210 of Human Rad51C.

Clonality

Polyclonal

Tissue Specificity

Expressed in a variety of tissues, with highest expression in testis, heart muscle, spleen and prostate.

Applications

WB, IHC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Essential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Part of the RAD21 paralog protein complexes BCDX2 and CX3 which act at different stages of the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 seems to act downstream of BRCA2 recruitment and upstream of RAD51 recruitment; CX3 seems to act downstream of RAD51 recruitment; both complexes bind predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway. Involved in RAD51 foci formation in response to DNA damage suggesting an involvement in early stages of HR probably in the invasion step. Has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHEK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Participates in branch migration and HJ resolution and thus is important for processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex. Part of a PALB2-scaffolded HR complex containing BRCA2 and which is thought to play a role in DNA repair by HR. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

42190 MW

Gene Name Synonym

DNA repair protein RAD51 homolog 3

Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion. DNA damage induces an increase in nuclear levels. Accumulates in DNA damage induced nuclear foci or RAD51C foci which is formed during the S or G2 phase of cell cycle. Accumulation at DNA lesions requires the presence of NBN/NBS1, ATM and RPA.

Protein Name

DNA repair protein RAD51 homolog 3

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2