Anti-Importin subunit beta-1 KPNB1 Antibody

About this product

Boster Bio Anti-Importin subunit beta-1 KPNB1 Antibody catalog # A01851. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A01851

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Importin subunit beta-1; Importin-90; Karyopherin subunit beta-1; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97; KPNB1; NTF97

Gene Name

KPNB1

UniProt

Q14974

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human KPNB1 (NP_002256.2) .

Clonality

Polyclonal

Applications

WB, IHC, ICC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

97170 MW

Gene Name Synonym

Importin subunit beta-1

Subcellular Location

Cytoplasm

Protein Name

Importin subunit beta-1

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2