- Catalog number
- A01905
- Size / count
- 100 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CaM Kinase IV (also known as CAM kinase-GR and CaMK IV) is a calcium/ calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. This kinase may be involved in the transcriptional regulation of microtubule dynamics. In vitro, CaMK IV phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. CaMK IV may also be involved in spermatogenesis and may play a role in the consolidation/ retention of hippocampus-dependent long-term memory. CaMK IV must be phosphorylated to be maximally active and is phosphorylated by CAMKK1 or CAMKK2. In addition autophosphorylation of the N-terminus is required for full activation. Autophosphorylation of Ser-336 allows the kinase to switch to a Ca (2+) /calmodulin-independent state. Most likely the kinase is inactivated by the serine/ threonine protein phosphatase 2A. CaMK IV is a monomer that is located within the cytoplasm and nucleus and substantial localization occurs in certain neuronal nuclei. In spermatids CaMK IV is associated with chromatin and the nuclear matrix. CaMK IV is also specifically expressed in epithelial ovarian cancer tissue.
- Synonyms
- CaM Kinase IV, CAM kinase 4, CAM kinase GR, CAM kinase IV, CaMK4, CaMKGR, Brain Ca (2+) calmodulin dependent protein kinase type IV, Calcium / calmodulin dependent protein kinase type 4 catalytic chain, CAMK, CAMK-GR, CAMKIV
- Gene Name
- CAMK4
- UniProt
- Q16566
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Cross Reactivity
- No cross reactivity with other proteins.
- Immunogen
- This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 300-325 of Human CaM Kinase IV protein.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.
- Applications
- ELISA, IHC, WB
- Field of Research
- Calcium Signaling, Calmodulin Pathway, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Neuroscience, Neurotransmission, Signal Transduction, Signaling Pathway
- Purification
- This antiserum is directed against human CaM Kinase IV protein. The product was delipidated, defibrinated followed by buffering and clarification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, and rat based on 100% homology for the immunogen sequence. Cross-reactivity with CaM Kinase IV homologues from other sources has not been determined.
- Concentration
- 70 mg/mL by Refractometry
- Form
- Lyophilized
- Reconstitution
- Restore with deionized water (or equivalent) for reconstitution volume of 100 μL
- Function
- Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4 (+) /CD8 (+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2) . Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs) . Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)
- Observed Molecular Weight
- 51926 MW
- Gene Name Synonym
- Calcium/calmodulin-dependent protein kinase type IV
- Subcellular Location
- Cytoplasm. Nucleus. Localized in hippocampal neuron nuclei. In spermatids, associated with chromatin and nuclear matrix (By similarity) .
- Prediction Reactivity
- Bovine, Chimpanzee, Canine
- Isotype
- Antiserum
- Contents
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
