Anti-POLK/Dna Polymerase Kappa Antibody

About this product

Boster Bio Anti-POLK/Dna Polymerase Kappa Antibody (Catalog # A01987) . Tested in ELISA, WB applications. This antibody reacts with Human.

Specifications

Catalog number

A01987

Size / count

100 µg

Dry ice

Yes

Hazardous

No

Discontinued

No

Background

POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair. POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity. POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.

Synonyms

POLK, DNA polymerase kappa, DINB homolog 1 (E. coli), DINB protein, DINB1, DINP, DNA damage inducible protein b, POLQ

Gene Name

POLK

UniProt

Q9UBT6

Host

Goat

Reactivity

Human

Immunogen

This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa) .

Clonality

Polyclonal

Tissue Specificity

Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.

Applications

ELISA, WB

Field of Research

DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling, RNA Polymerase, Transcription

Purification

This affinity purified antibody is directed against human POLK protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. Cross-reactivity with POLK protein from mouse is expected, as this sequence is highly homologous in human and mouse. Expect reactivity with multiple splice variants of this protein. Cross-reactivity with POLK homologues from other sources has not been determined.

Concentration

1.4 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

98809 MW

Gene Name Synonym

DNA polymerase kappa

Subcellular Location

Nucleus. Detected throughout the nucleus and at replication foci.

Isotype

IgG

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide