Anti-HR23B RAD23B Antibody

About this product

Boster Bio Anti-HR23B RAD23B Antibody (Catalog # A02174) . Tested in ELISA, WB applications. This antibody reacts with Human.

Specifications

Catalog number

A02174

Size / count

100 µg

Dry ice

Yes

Hazardous

No

Discontinued

No

Background

HR23B (also known as UV excision repair protein RAD23 homolog B, XP-C repair complementing complex 58 kDa protein and p58) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER) . This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Synonyms

HR23B, hHR23B, mHR23B, p58, RAD23 (S. cerevisiae) homolog B, UV excision repair protein RAD23 homolog B, XP-C repair-complementing complex 58 kDa protein

Gene Name

RAD23B

UniProt

P54727

Host

Goat

Reactivity

Human

Immunogen

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 155-180 of human HR23B protein.

Clonality

Polyclonal

Applications

ELISA, WB

Purification

This is an affinity-purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human HR23B protein. Sequence homology as assessed by BLAST indicated 100% homology for this protein from human, dog, chimpanzee and S. cerevisiae. Cross-reactivity with HR23B protein from mouse and rat may also occur as sequence homology varies by one amino acid residue in this sequence by BLAST analysis. Reactivity with HR23B protein from other sources is not known.

Concentration

1.1 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome.

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

43171 MW

Gene Name Synonym

UV excision repair protein RAD23 homolog B

Subcellular Location

Nucleus. Cytoplasm. The intracellular distribution is cell cycle dependent. Localized to the nucleus and the cytoplasm during G1 phase. Nuclear levels decrease during S- phase; upon entering mitosis, relocalizes in the cytoplasm without association with chromatin.

Isotype

IgG

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide