Anti-Collagen Type VI COL6A1 Antibody

About this product

Boster Bio Anti-Collagen Type VI COL6A1 Antibody (Catalog # A02226) . Tested in Dot blot, IHC applications. This antibody reacts with Human, Bovine.

Specifications

Catalog number

A02226

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Boster extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

Synonyms

Collagen Type VI, Collagen alpha-1 (VI) chain, Collagen VI, Human mRNA for collagen VI alpha 1 C terminal globular domain

Gene Name

COL6A1-COL6A3

UniProt

P12109

Host

Rabbit

Reactivity

Bovine, Human

Immunogen

Collagen Type VI from human and bovine placenta

Clonality

Polyclonal

Applications

ELISA, IHC, IP, WB

Field of Research

Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction

Purification

Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Concentration

1.01 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. (Ship on wet ice.)

Isotype

IgG

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide